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CD3 抗体

This anti-CD3 antibody is a 小鼠 单克隆 antibody detecting CD3 in FACS, IP, IHC, IHC (fro), WB 和 ICC. Suitable for 人 和 小鼠.
产品编号 ABIN490154
发货至: 中国

Quick Overview for CD3 抗体 (ABIN490154)

抗原

See all CD3 抗体
CD3

适用

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人, 小鼠

宿主

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小鼠

克隆类型

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单克隆

标记

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This CD3 antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB), Immunocytochemistry (ICC)

克隆位点

APA1-1
  • 特异性

    The mouse monoclonal antibody APA1/1 recognizes an activation-dependent intracellular epitope of CD3 epsilon. Exposure of the epitope precedes CD3 phosphorylation and recruitment and activation of ZAP70, which initiates the signaling cascade produced by T-cell activation. APA1/1 provides the earliest known marker for TCR-mediated T cell activation.

    纯化方法

    Protein A purified

    免疫原

    Purified human CD3 proteins isolated from thymus.

    Type of Immunogen: Purified protein

    亚型

    IgG1
  • 应用备注

    Approved: Flo, ICC, IHC, IHC-Fr, IP, WB

    Usage: Flow Cytometry: Positive control - Human T cells stimulated with anti-CD3 (MEM-57) antibody (1 μg/mL). Sample preparation:. At the end of stimulation of T cells, perform staining of surface markers (if required) in PBS + 0.1 % BSA for 20 min. on ice. Wash

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS, 15 mM sodium azide,  pH 7.4

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    avoid freeze thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
  • 抗原

    CD3

    别名

    CD3

    途径

    TCR Signaling, Ubiquitin Proteasome Pathway
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